MyH7 antisense long non-coding RNA promotes the formation of slow-twitch fibers through stabilizing MyH7 mRNA.
MyH7反义长链非编码RNA通过稳定MyH7 mRNA促进慢肌纤维的形成。
Haifeng Dan, Jia Hao, Tongtong Zhang, Saishaung Ying, Rui Cai, Weijun Pang (2026) MyH7 antisense long non-coding RNA promotes the formation of slow-twitch fibers through stabilizing MyH7 mRNA. Int J Biol Macromol (IF: 8.5) 2 区 151984
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Abstract
Long non-coding RNA (lncRNA) can regulate mRNA stability by directly binding to mRNA, exerting a significant regulatory effect on muscle growth and development. Improving pork quality is a key objective in breeding superior pig breeds, where the proportion of slow-twitch fibers is intimately linked to meat quality attributes. In this study, we found that a cross-species conserved MyH7 antisense (AS) lncRNA was significantly more highly expressed in the longissimus thoracis (LT) muscle of the obese-type Bamei pigs, suggesting its potential role in myofiber type switching. Overexpression of MyH7 AS lncRNA in porcine skeletal muscle primary cells and C2C12 myoblasts promoted the formation of slow-twitch myotubes and inhibited the expression of fast myosin heavy chain protein. Mechanistic investigation revealed that MyH7 AS lncRNA enhances the stability of MyH7 mRNA by forming a double-stranded structure with it, thereby facilitating the generation of slow-twitch fibers. Further in vivo experiments demonstrated that intramuscular injection of AAV9-mediated MyH7 AS lncRNA overexpression vectors into mice elevated the proportion of slow-twitch fibers and enhanced exercise endurance. These results provide a theoretical basis for improving pork quality by regulating muscle fiber types.Copyright © 2026. Published by Elsevier B.V.
长链非编码RNA（lncRNA）可通过直接结合mRNA来调控mRNA的稳定性，从而对肌肉的生长发育发挥显著的调控作用。提高猪肉品质是培育优良猪种的关键目标，其中慢肌纤维的比例与肉质密切相关。本研究发现，一种跨物种保守的MyH7反义（AS）lncRNA在肥胖型巴美猪的背最长肌（LT）中显著高表达，提示其可能参与肌纤维类型转换。在猪骨骼肌原代细胞和C2C12成肌细胞中过表达MyH7 AS lncRNA可促进慢肌肌管的形成，并抑制快肌肌球蛋白重链的表达。机制研究表明，MyH7 AS lncRNA通过与MyH7 mRNA形成双链结构来增强其稳定性，从而促进慢肌纤维的生成。进一步的体内实验表明，将AAV9介导的MyH7 AS lncRNA过表达载体肌内注射到小鼠体内，可提高慢肌纤维的比例并增强其运动耐力。这些结果为通过调控肌肉纤维类型来改善猪肉品质提供了理论依据。版权所有 © 2026。由Elsevier BV出版。
Links
http://www.ncbi.nlm.nih.gov/pubmed/41980688http://dx.doi.org/10.1016/j.ijbiomac.2026.151984
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